Cold transduction by inhibition of a background potassium conductance in rat primary sensory neurones

被引:103
作者
Reid, G [1 ]
Flonta, ML [1 ]
机构
[1] Univ Bucharest, Fac Biol, Dept Anim Physiol & Biophys, Bucharest 76201, Romania
关键词
cold; thermoreceptor; sensory transduction; primary sensory neurone; dorsal root ganglion; background potassium current; Na+/K+ ATPase;
D O I
10.1016/S0304-3940(00)01694-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Transduction in cutaneous cold receptors is poorly understood at present. We have studied this question using dorsal root ganglion (DRG) neurones in primary culture as a model of the otherwise inaccessible receptor terminal. Whole-cell recordings during cooling from 32 to 20 degreesC revealed a large depolarization (> 8mV) in 22 of 88 DRG neurones (25%), sometimes accompanied by action potentials. In cold-sensitive neurones cooling inhibited a time-independent background K+ current (I-cold) which was resistant to tetraethylammonium and 4-amifiopyridine. Ouabain elicited a substantially smaller depolarization than cooling, and no action potentials. We conclude that excitation by cooling in this model is primarily due to inhibition of I-cold and that the previously suggested role of the Na+/K+ adenosine triphosphatase is secondary. We suggest that I-cold may underlie cold transduction in cutaneous thermoreceptors. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:171 / 174
页数:4
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