Characterization of HIV-1 Tat proteins mutated in the transactivation domain for prophylactic and therapeutic application

被引：15

作者：

Betti, M

Voltan, R

Marchisio, M

Mantovani, I

Boarini, C

Nappi, F

Ensoli, B

Caputo, A

机构：

[1] Univ Ferrara, Dept Expt & Diagnost Med, I-44100 Ferrara, Italy

[2] Univ Ferrara, Dept Morphol & Embryol, I-44100 Ferrara, Italy

[3] Ist Super Sanita, Virol Lab, I-00161 Rome, Italy

来源：

VACCINE | 2001年 / 19卷 / 25-26期

关键词：

HIV-1; Tat mutants; vaccine;

D O I：

10.1016/S0264-410X(01)00067-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous work from our group showed that genetic immunization of mice with HIV-1 tat genes (tat(22) and tat(22/37)), encoding Tat proteins mutated in the transactivation domain and lacking Tat-transactivating activity, evoke an immune response to wild-type Tat, both humoral and cellular. In the present work we report that the mutated Tat proteins localize within the cells, are released and taken up by the cells in a fashion similar to wild-type Tat. Moreover, the exogenous mutated Tat proteins interfere with the These results support the notion that tat(22) and tat(22/37) genes may represent good candidates for the development of an anti-HIV-1 vaccine, especially for HIV-1 infected patients. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：3408 / 3419

页数：12

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