The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems

被引:19
作者
Kudryasheva, NS
Kudinova, IY
Esimbekova, EN
Kratasyuk, VA
Stom, DI
机构
[1] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[2] Irkutsk State Univ, Biol Res Inst, Irkutsk 664003, Russia
关键词
D O I
10.1016/S0045-6535(98)00218-5
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Kinetics of the triple bioluminescent enzyme system: alcohol dehydrogenase - NADH:FMN-oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD(+)/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:751 / 758
页数:8
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