A cardioactive peptide from the southern armyworm, Spodoptera eridania

被引：30

作者：

Furuya, K

Hackett, M

Cirelli, MA

Schegg, KM

Wang, HL

Shabanowitz, J

Hunt, DF

Schooley, DA ^{[1
]}

机构：

[1] Univ Nevada, Dept Biochem, Reno, NV 89557 USA

[2] Genentech Inc, San Francisco, CA 94080 USA

[3] Univ Washington, Dept Med Chem, Seattle, WA 98195 USA

[4] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA

[5] Bruker Daltonics Inc, Billerica, MA 01821 USA

来源：

PEPTIDES | 1999年 / 20卷 / 01期

关键词：

amino acid sequence; myotropic peptide; insect neuropeptide; Manduca heart assay; paralytic peptide; juvenile hormone esterase activity-repressive and growth-blocking peptide; plasmatocyte-spreading peptide;

D O I：

10.1016/S0196-9781(98)00148-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cardioactive peptide was isolated from extracts of whole heads of the southern armyworm, Spodoptera eridania. This peptide has the sequence ENFAVGCTPGYQRTADGRCKPTF (M-r = 2516.8), determined from both Edman sequencing and tandem mass spectrometry in combination with off-line micropreparative capillary liquid chromatography. This peptide, termed Spoer-CAP(23), has excitatory effects on a semi-isolated heart from larval Manduca sexta, causing an inotropic effect at low concentrations of peptide and chronotropic and inotropic effects at high doses. The threshold concentration for stimulatory effects of the synthetic peptide on the semi-isolated heart was about 1 nM, suggesting a physiological role as a neuropeptide. (C) 1999 by Elsevier Science Inc.

引用

页码：53 / 61

页数：9

共 45 条

[1]

ARNOTT D, 1992, 40 ASMS WASH

[2] ALPHA-CYANO-4-HYDROXYCINNAMIC ACID AS A MATRIX FOR MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY [J].