Application of high-performance liquid chromatography with photodiode array detection to the metabolic profiling of plant isoprenoids

被引：228

作者：

Fraser, PD ^{[1
]}

Pinto, MES ^{[1
]}

Holloway, DE ^{[1
]}

Bramley, PM ^{[1
]}

机构：

[1] Univ London, Royal Holloway, Sch Biol Sci, Egham TW20 0EX, Surrey, England

来源：

PLANT JOURNAL | 2000年 / 24卷 / 04期

关键词：

high-performance liquid chromatography; isoprenoids; metabolic profiling;

D O I：

10.1111/j.1365-313X.2000.00896.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The application of high-performance liquid chromatography (HPLC) using a C-30 reverse-phase stationary matrix has enabled the simultaneous separation of carotenes, xanthophylls, ubiquinones, tocopherols and plastoquinones in a single chromatogram. Continuous photodiode array (PDA) detection ensured identification and quantification of compounds upon elution. Applications of the method to the characterization of transgenic and mutant tomato varieties with altered isoprenoid content, biochemical screening of Arabidopsis thaliana, and elucidation of the modes of action of bleaching herbicides are described to illustrate the versatility of the procedure.

引用

页码：551 / 558

页数：8

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