Mammalian phospholipase D: Phosphatidylethanolamine as an essential component

被引：36

作者：

Nakamura, SI ^{[1
]}

Kiyohara, Y ^{[1
]}

Jinnai, H ^{[1
]}

Hitomi, T ^{[1
]}

Ogino, C ^{[1
]}

Yoshida, K ^{[1
]}

Nishizuka, Y ^{[1
]}

机构：

[1] KOBE UNIV,BIOSIGNAL RES CTR,KOBE,HYOGO 657,JAPAN

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 09期

关键词：

D O I：

10.1073/pnas.93.9.4300

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bovine kidney phospholipase D (PLD) was assayed by measuring the formation of phosphatidylethanol from added radioactive phosphatidylcholine (PtdCho) in the presence of ethanol, guanosine 5'-[gamma-thio]triphosphate, ammonium sulfate, and cytosol factor that contained small GTP-binding regulatory proteins. The PLD enzyme associated with particulate fractions was solubilized by deoxycholate and partially purified by chromatography on a heparin-Sepharose column. This PLD preferentially used PtdCho as substrate. After purification, the enzyme per se showed little or practically no activity but required an additional factor for the enzymatic reaction. This factor was extracted with chloroform/methanol directly from particulate fractions of various tissues, including kidney, liver, and brain, and identified as phosphatidylethanolamine (PtdEtn), although this phospholipid did not serve as a good substrate. Plasmalogen-rich PtdEtn, dioleoyl-PtdEtn, and L-alpha-palmitoyl-beta-linoleoyl-PtdEtn were effective, but dipalmitoyl-PtdEtn was inert. Sphingomyelin was 30% as active as PtdEtn. The results suggest that mammalian PLD reacts nearly selectively with PtdCho in the form of mixed micelles or membranes with other phospholipids, especially PtdEtn.

引用

页码：4300 / 4304

页数：5

共 15 条

[1] THE REGULATION AND CELLULAR FUNCTIONS OF PHOSPHATIDYLCHOLINE HYDROLYSIS [J].