Celiac disease screening by immunochromatographic visual assays:: Results of a multicenter study

被引:18
作者
Baviera, Luis Carlos Blesa
Aliaga, Ester Donat
Ortigosa, Luis
Litwin, Nestor
Pena-Quintana, Luis
Mendez, Virginia
Gonzalez, M. Victoria
机构
[1] La Fe Childrens Hosp, Pediat Gastroenterol Unit, Valencia, Spain
[2] Nuestra Sra Candelaria Univ Hosp, Pediat Gastroenterol Unit, Tenerife, Spain
[3] Inst Invest Med Dr Alfredo Lanari, Pediat Gastroenterol Unit, Buenos Aires, DF, Argentina
[4] Canary Univ Hosp, Pediat Gastroenterol Unit, Las Palmas Gran Canaria, Spain
[5] Pereira Rosell Hosp, Pediat Gastroenterol Unit, Montevideo, Uruguay
[6] Pereira Rosell Hosp, Clin Immunol Lab, Montevideo, Uruguay
[7] Doce Octubre Hosp, Pediat Gastroenterol Unit, Madrid, Spain
[8] Natl Biotechnol Ctr, Madrid, Spain
关键词
celiac disease; immunochromatographic visual method; screening;
D O I
10.1097/MPG.0b013e31814794b9
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Objective: To assay the efficiency for celiac disease (CD) screening of 2 immunochromatographic visual stick assays based on human recombinant tissue transglutaminase (tTG). One was the antitissue transglutaminase antibodies (AtTGA) stick for IgA/G antibodies to tTG detection, the other was the AtTGA/antigliadin antibodies (AGA) stick for I-A antibodies for tTG and/or gliadins. Patients and Methods: In a prospective multicenter study, 4 pediatric gastroenterology units from Spain and 2 from Latin America enrolled 72 control children with a normal small bowel mucosa and 1] 3 untreated patients with CD with Marsh type 3 lesions. Results: Evaluation of results by the gastroenterologists and by 2 independent observers at the coordination center showed a remarkably low interobserver variability. For the AtTGA stick, sensitivity was 96.5% and specificity was 98.6%. The AtTGA/AGA stick displayed a sensitivity of 94.5% and a specificity of 98.6% for AtTGA and a sensitivity of 63.1 % and a specificity of 95.2% for AGA. The highest efficiency and positive likelihood ratio was obtained for the AtTGA stick, higher than for IgA AtTGA by enzyme-linked immunosorbent assay. One additional advantage was that previous investigation of total serum IgA levels could be eluded. The IgA AtTGA/AGA stick, with an efficiency of 95.1 %, compared with 89.2% when the combined results of the 2 enzyme-linked immunosorbent assays were considered, turned out to be an excellent diagnostic tool for infants with no IgA deficiency. Conclusion: These 2 assays are extremely efficient for CID screening, by combining a high diagnostic accuracy with the simplicity and rapidity of visual methods.
引用
收藏
页码:546 / 550
页数:5
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