Active repression and E2F inhibition by pRB are biochemically distinguishable

被引:25
作者
Ross, JF
Näär, A
Cam, H
Gregory, R
Dynlacht, BD [1 ]
机构
[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA
[2] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Berkeley, CA 94720 USA
关键词
pRB; E2F; transcriptional repression; chromatin;
D O I
10.1101/gad.858501
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
To understand mechanistically how pRB represses transcription, we used a reconstituted transcription assay and compared PRE activity on naked versus chromatin templates. Surprisingly, when pRB was directly recruited to a naked template, no transcriptional repression was observed. However, we observed active repression when the same promoter was assembled into chromatin. Histone deacetylases do not appear to play a role in this observed repression. Further experiments showed repression could occur after preinitiation complex assembly, in contrast with pRB inhibition of E2F, suggesting discrete mechanisms by which pRB directly inhibits an activator such as E2F or actively represses proximally bound transcription factors.
引用
收藏
页码:392 / 397
页数:6
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