Polymorphisms of GA/GT microsatellite loci from Anguilla japonica

被引：29

作者：

Tseng, MC

Chen, CA

Kao, HW

Tzeng, WN

Lee, SC ^{[1
]}

机构：

[1] Acad Sinica, Inst Zool, Taipei 11529, Taiwan

[2] Natl Taiwan Univ, Dept Zool, Taipei 10764, Taiwan

来源：

MARINE BIOTECHNOLOGY | 2001年 / 3卷 / 03期

关键词：

microsatellite; catadromous; Anguilla japonica; polymorphism; genotype diversity;

D O I：

10.1007/s101260000076

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Six novel microsatellite loci, containing (GA)(15-17) or (GT)(10-19) perfect tandem repeats, were isolated and characterized for the catadromous eel Anguilla japonica. The allelic size of the 6 loci ranged from 79 to 226 bp in length. All loci were polymorphic with a mean number of 14.7 alleles per locus and a mean heterozygosity of 0.67, suggesting higher polymorphism than that of freshwater and anadromous fishes, but lower than that of marine fishes. Genotype diversity of the 6 loci ranged from 0.22 to 0.61 with a mean value of approximately 0.5. Cross-species amplification showed that 5 of the 6 microsatellite primers proved to be useful in addressing questions of population genetics for all Anguilla species.

引用

页码：275 / 280

页数：6

共 19 条

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Beacham TD, 1998, J FISH BIOL, V52, P665, DOI 10.1111/j.1095-8649.1998.tb00811.x

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