SP1-mediated upregulation of lncRNA LMCD1-AS1 functions a ceRNA for miR-106b-5p to facilitate osteosarcoma progression

被引：25

作者：

He, Jia-wen ^{[1
]}

Li, De-jian ^{[1
]}

Zhou, Jian-hua ^{[1
]}

Zhu, Ya-long ^{[1
]}

Yu, Bao-qing ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Pudong Hosp, Dept Orthoped, Pudong Med Ctr, 28 Gongwei Rd, Shanghai, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2020年 / 526卷 / 03期

关键词：

LncRNA LMCD1-AS1; SP1; miR-106b-5p; Osteosarcoma; Metastasis; Biomarker; RNA; MICRORNA; CELLS;

D O I：

10.1016/j.bbrc.2020.03.151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Growing studies have indicated the involvements of long noncoding RNAs (lncRNAs) in the initiation and progression of various tumors. We aimed to investigated the role of lncRNA LMCD1 antisense RNA 1 (LMCD1-AS1) in osteosarcoma development. We found that LMCD1-AS1 and SP1 were highly expressed in osteosarcoma tissues and cell lines. High levels of LMCD1-AS1 were correlated with positively metastasis and poor clinical prognosis. Moreover, we showed that SP1 can bind to the promoter region of LMCD1-AS1, resulting in its overexpression in osteosarcoma. Functionally, silencing of LMCD1-AS1 suppressed the proliferation, migration, invasion and EMT progress of osteosarcoma cells. Mechanistic studies revealed that LMCD1-AS1 was a sponge of miR-106b-5p activity. LMCD1-AS1 modulated survival of osteosarcoma via targeting miR-106b-5p. Overall, we firstly indicated that LMCD1-AS1 overexpression contributes to osteosarcoma development and poor clinical outcome, suggesting that LMCD1-AS1 may be a novel diagnostic and prognostic biomarker for osteosarcoma and a target for osteosarcoma therapy. (C) 2020 Elsevier Inc. All rights reserved.

引用

页码：670 / 677

页数：8

共 27 条

[1]

Recent Advances in Mechanobiology of Osteosarcoma [J].