Analysis of the stress resistance of commercial wine yeast strains

被引:92
作者
Carrasco, P
Querol, A
del Olmo, M
机构
[1] CSIC, Inst Agroquim & Tecnol Alimentos, E-46010 Valencia, Spain
[2] Univ Valencia, Fac Ciencies Biol, Dept Bioquim & Biol Mol, Valencia, Spain
关键词
stress; wine yeasts; Saccharomyces cerevisiae; HSP12; HSP104; alcoholic fermentation; gene expression;
D O I
10.1007/s002030100289
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Alcoholic fermentation is an essential step in wine production that is usually conducted by yeasts belonging to the species Saccharomyces cerevisiae. The ability to carry out vinification is largely influenced by the response of yeast cells to the stress conditions that affect them during this process. In this work, we present a systematic analysis of the resistance of 14 commercial S. cerevisiae wine yeast strains to heat shock, ethanol, oxidative, osmotic and glucose starvation stresses. Significant differences were found between these yeast strains under certain severe conditions, Vitilevure Pris Mouse and Lalvin T73 being the most resistant strains, while Fermi-blanc arom SM102 and UCLM S235 were the most sensitive ones. Induction of the expression of the HSP12 and HSP104 genes was analyzed. These genes are reported to be involved in the tolerance to several stress conditions in laboratory yeast strains. Our results indicate that each commercial strain shows a unique pattern of gene expression, and no clear correlation between the induction levels of either gene and stress resistance under the conditions tested was found. However, the increase in mRNA levels in both genes under heat shock indicates that the molecular mechanisms involved in the regulation of their expression by stress function in all of the strains.
引用
收藏
页码:450 / 457
页数:8
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