Molecular Characterization of Feline Infectious Peritonitis Virus Strain DF-2 and Studies of the Role of ORF3abc in Viral Cell Tropism

被引：62

作者：

Balint, Adam ^{[1
,5
]}

Farsang, Attila ^{[2
,5
]}

Zadori, Zoltan ^{[3
,5
]}

Hornyak, Akos ^{[1
,5
]}

Dencso, Laszlo ^{[1
]}

Almazan, Fernando ^{[4
]}

Enjuanes, Luis ^{[4
]}

Belak, Sandor ^{[5
,6
]}

机构：

[1] Agr Off Vet Diagnost Directorate, Dept Virol, Budapest, Hungary

[2] Agr Off Directorate Vet Med Prod, Budapest, Hungary

[3] Hungarian Acad Sci, Vet Med Res Inst, Agr Res Ctr, H-1581 Budapest, Hungary

[4] Campus Univ Autonoma Madrid, Dept Mol & Cell Biol, Ctr Nacl Biotecnol CNB CSIC, Madrid, Spain

[5] Swedish Univ Agr Sci, Dept Biomed Sci & Vet Publ Hlth, Uppsala, Sweden

[6] Natl Vet Inst, Dept Virol Immunobiol & Parasitol, S-75007 Uppsala, Sweden

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 11期

关键词：

GENOMIC RNA SEQUENCE; CORONAVIRUS INFECTIONS; 3A PROTEIN; IMMUNODEFICIENCY-VIRUS; TRANSMEMBRANE TOPOLOGY; GENETIC-ANALYSIS; PREVALENCE; SEROPREVALENCE;

D O I：

10.1128/JVI.00189-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The full-length genome of the highly lethal feline infectious peritonitis virus (FIPV) strain DF-2 was sequenced and cloned into a bacterial artificial chromosome (BAC) to study the role of ORF3abc in the FIPV-feline enteric coronavirus (FECV) transition. The reverse genetic system allowed the replacement of the truncated ORF3abc of the original FIPV DF-2 genome with the intact ORF3abc of the canine coronavirus (CCoV) reference strain Elmo/02. The in vitro replication kinetics of these two viruses was studied in CrFK and FCWF-4 cell lines, as well as in feline peripheral blood monocytes. Both viruses showed similar replication kinetics in established cell lines. However, the strain with a full-length ORF3 showed markedly lower replication of more than 2 log(10) titers in feline peripheral blood monocytes. Our results suggest that the truncated ORF3abc plays an important role in the efficient macrophage/monocyte tropism of type II FIPV.

引用

页码：6258 / 6267

页数：10

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