Role of the endometrial tripod interieukin-18,-15, and-12 in inadequate uterine receptivity in patients with a history of repeated in vitro fertilization- embryo transfer failure

被引:124
作者
Lédée-Bataille, N
Bonnet-Chea, K
Hosny, G
Dubanchet, SY
Frydman, R
Chaouat, G
机构
[1] Hop Antoine Beclere, INSERM U131, Equipe Cytokines & Relat Materno Foetale, Clamart, France
[2] Hop Antoine Beclere, Serv Gynecol Obstet, EA 3538, Clamart, France
关键词
endometrium; interleukin-12; interleukin-18; real-time PCR; IVF-embryo transfer;
D O I
10.1016/j.fertnstert.2004.11.021
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To document in the endometrium the correlation among the interleukin (IL)-12, -15, and -18 mRNA and the correlation between cytokine levels, vascular status, and endometrial natural killer (NK) cell count in the context of recurrent implantation failure. Design: A pilot study. Setting: Department of Reproductive Immunology. Patient(s): Women who failed to become pregnant after repeated IVF-embryo transfer and fertile control subjects. Intervention(s): Ultrasonic evaluation and endometrial biopsy in luteal phase. Main Outcome Measure(s): Uterine artery Doppler, count of uterine CD56 bright cells/field, and quantification by real-time polymerase chain reaction (PCR) to monitor IL-12 family (IL-12p40, IL-12p35, EBI3, IL-23), the IL-18 system (IL-18, IL-18R, IL18BP), and the IL-15 mRNA ratio. Result(s): The uterine artery Doppler and the CD56 bright cell counts were significantly different in fertile and infertile patients. The mean uterine artery pulsatility index correlated significantly negatively with the IL-18/actin ratio suggesting a defect of the cytokine-dependent vascular remodeling pathway. The number of uterine CD56 bright cells was significantly correlated with the IL-15/actin and IL-18/IL-18BP ratios. Thus, IL-18 and IL-15 seems to be involved in the local recruitment and the activation of uterine natural killer (uNK) cells. IL-18 was itself correlated with IL-15 and IL-12, suggesting a local control of uNK cells activation. Conclusion(s): The assessment of the tripod IL-12/-15/-18 shows distinct immune-related mechanisms that are involved in the broader context of inadequate uterine receptivity. (c) 2005 by American Society for Reproductive Medicine.
引用
收藏
页码:598 / 605
页数:8
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