High-sensitivity sapphire cells for high pressure NMR spectroscopy on proteins

被引：38

作者：

Arnold, MR ^{[1
]}

Kalbitzer, HR ^{[1
]}

Kremer, W ^{[1
]}

机构：

[1] Univ Regensburg, Inst Biophys & Phys Biochem, D-93040 Regensburg, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2003年 / 161卷 / 02期

关键词：

high pressure; NMR sectroscopy; proline cis-trans isomerisation; glass cell method; sapphire;

D O I：

10.1016/S1090-7807(02)00179-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High pressure NMR spectroscopy is a most exciting method for studying the structural anisotropy and conformational dynamics of proteins. The restricted volume of the high pressure glass cells causes a poor signal to noise ratio which up to now renders the application of most of the multidimensional NMR experiments impossible. The method presented here using high strength single crystal sapphire cells doubles the signal-to-noise ratio and allows to perform high pressure NMR measurements more easily. As a first application the difference of partial molar volumes caused by cis-trans-isomerisation of a prolyl peptide bond in the tetrapeptide Gly-Gly-Pro-Ala could be determined as 0.25 ml mol(-1) at 305 K. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：127 / 131

页数：5

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