A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects

被引:147
作者
Costabile, Adele [1 ]
Kolida, Sofia [1 ]
Klinder, Annett [1 ]
Gietl, Eva [2 ]
Baeuerlein, Michael [3 ]
Frohberg, Claus [2 ]
Landschuetze, Volker [2 ]
Gibson, Glenn R. [1 ]
机构
[1] Univ Reading, Sch Food & Nutr Sci, Reading RG6 6AP, Berks, England
[2] Bayer BioSci GmbH, D-14473 Potsdam, Germany
[3] Bayer Cropsci AG, D-40789 Monheim, Germany
关键词
Prebiotics; Bifidogenic effect; Gas production; Intestinal microflora; IN-VITRO FERMENTATION; RNA-BASED PROBES; FECAL BIFIDOBACTERIA; FRUCTO-OLIGOSACCHARIDES; CHICORY INULIN; OLIGONUCLEOTIDE PROBES; BACTERIAL-POPULATIONS; JERUSALEM-ARTICHOKE; SITU HYBRIDIZATION; HUMAN FECES;
D O I
10.1017/S0007114510001571
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
There is growing interest in the use of inulins as substrates for the selective growth of beneficial gut bacteria such as bifidobacteria and lactobacilli because recent studies have established that their prebiotic effect is linked to several health benefits. In the present study, the impact of a very-long-chain inulin (VLCI), derived from globe artichoke (Cynara scolymus), on the human intestinal microbiota compared with maltodextrin was determined. A double-blind, cross-over study was carried out in thirty-two healthy adults who were randomised into two groups and consumed 10 g/d of either VLCI or maltodextrin, for two 3-week study periods, separated by a 3-week washout period. Numbers of faecal bifidobacteria and lactobacilli were significantly higher upon VLCI ingestion compared with the placebo. Additionally, levels of Atopobium group significantly increased, while Bacteroides-Prevotella numbers were significantly reduced. No significant changes in faecal SCFA concentrations were observed. There were no adverse gastrointestinal symptoms apart from a significant increase in mild and moderate bloating upon VLCI ingestion. These observations were also confirmed by in vitro gas production measurements. In conclusion, daily consumption of VLCI extracted from globe artichoke exerted a pronounced prebiotic effect on the human faecal microbiota composition and was well tolerated by all volunteers.
引用
收藏
页码:1007 / 1017
页数:11
相关论文
共 68 条
[1]  
Alles MS, 1999, AM J CLIN NUTR, V69, P980
[2]   COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].
AMANN, RI ;
BINDER, BJ ;
OLSON, RJ ;
CHISHOLM, SW ;
DEVEREUX, R ;
STAHL, DA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925
[3]  
Blaut Michael, 2002, European Journal of Nutrition, V41, P11
[4]   Supplementation of a bovine milk formula with an oligosaccharide mixture increases counts of faecal bifidobacteria in preterm infants [J].
Boehm, G ;
Lidestri, M ;
Casetta, P ;
Jelinek, J ;
Negretti, F ;
Stahl, B ;
Marini, A .
ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION, 2002, 86 (03) :178-181
[5]   Short-chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans [J].
Bouhnik, Y ;
Vahedi, K ;
Achour, L ;
Attar, A ;
Salfati, J ;
Pochart, P ;
Marteau, P ;
Flourié, B ;
Bornet, F ;
Rambaud, JC .
JOURNAL OF NUTRITION, 1999, 129 (01) :113-116
[6]  
BOUHNIK Y, 1994, GASTROENTEROLOGY, V106, pA598
[7]   The capacity of short-chain fructo-oligosaccharides to stimulate faecal bifidobacteria: a dose-response relationship study in healthy humans [J].
Bouhnik, Yoram ;
Raskine, Laurent ;
Simoneau, Guy ;
Paineau, Damien ;
Bornet, Francis .
NUTRITION JOURNAL, 2006, 5 (1)
[8]  
Brandt L.A., 2001, Prepared Foods, V170, pNS7
[9]   Effect of consumption of a ready-to-eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers [J].
Brighenti, F ;
Casiraghi, MC ;
Canzi, E ;
Ferrari, A .
EUROPEAN JOURNAL OF CLINICAL NUTRITION, 1999, 53 (09) :726-733
[10]   Digestive tolerance of inulin-type fructans: a double-blind, placebo-controlled, cross-over, dose-ranging, randomized study in healthy volunteers [J].
Bruhwyler, Jacques ;
Carreer, Fabienne ;
Demanet, Etienne ;
Jacobs, Heidi .
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 2009, 60 (02) :165-175