Kanamycin susceptibility testing of Mycobacterium tuberculosis using mycobacterium growth indicator tube and a colorimetric method

被引:31
作者
Bastian, I
Rigouts, L
Palomino, JC
Portaels, F
机构
[1] Inst Med & Vet Sci, Rundle Mall, SA 5000, Australia
[2] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
关键词
D O I
10.1128/AAC.45.6.1934-1936.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 mug/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 mug/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 pg of kanamycin/ml. Both systems provided results within an average of 1 week.
引用
收藏
页码:1934 / 1936
页数:3
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