Agonist stimulation of B1 and B2 kinin receptors causes activation of the MAP kinase signaling pathway, resulting in the translocation of AP-1 in HEK 293 cells

In response to bradykinin, phosphorylated MAP kinases (ERK-1 and ERK-2) were abundantly increased in HEK 293 cells, which overexpress the rat B-2 kinin receptor. In a similar may des-Arg(9)-bradykinin stimulation of B-1 kinin receptor-overexpressing HEK 293 cells caused activation of the same species of MAP kinase, Furthermore, nuclear translocation of transcription factor AP-1 was also found in the cells after stimulation with either agonist, PD98059, a MAP kinase kinase (MEK-1) inhibitor, blocked the agonist-induced AP-1 translocation as well as the phosphorylation of the MAP kinases, This communication provides the first evidence for both B-1 and B-2 kinin receptors mediating the MAP kinase signaling pathway to activate AP-1, (C) 1998 Federation of European Biochemical Societies.