Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes

被引：77

作者：

Bellin, T

Pulz, M

Matussek, A

Hempen, HG

Gunzer, F

机构：

[1] Hannover Med Sch, Inst Med Microbiol, D-30625 Hannover, Germany

[2] Niedersach Landesgesundheitsamt, D-30449 Hannover, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 01期

关键词：

D O I：

10.1128/JCM.39.1.370-374.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

引用

页码：370 / 374

页数：5

共 18 条

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