Genetic analysis of two bile salt hydrolase activities in Lactobacillus acidophilus NCFM

被引:105
作者
McAuliffe, O
Cano, RJ
Klaenhammer, TR
机构
[1] N Carolina State Univ, Dept Food Sci, SE Dairy Foods Res Ctr, Raleigh, NC 27695 USA
[2] Calif Polytech State Univ San Luis Obispo, Environm Biotechnol Inst, San Luis Obispo, CA 93407 USA
关键词
D O I
10.1128/AEM.71.8.4925-4929.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.
引用
收藏
页码:4925 / 4929
页数:5
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