Crosslinking CD81 results in activation of TCRγδ T cells

被引:21
作者
Tseng, CTK [1 ]
Miskovsky, E
Klimpel, GR
机构
[1] Univ Texas, Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA
[2] Univ Texas, Med Branch, Dept Gastroenterol, Galveston, TX 77555 USA
关键词
cytokine; T cells; IFN-gamma; T cell activation;
D O I
10.1006/cimm.2000.1744
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
CD81 is expressed on most cells and is associated with other glycoproteins, including CD4 and CD8, to form multimolecular membrane complexes. Crosslinking of CD81 on TCR alpha beta (+) T cells results in costimulatory signals that have been proposed to be mediated via CD4 or CD8, In this study, we show that CD81 is also expressed on TCR gamma delta (+)CD4(-)CD8(-) T cells. CD81 crosslinking greatly enhanced anti-CD3 activation of both TCR alpha beta (+) (CD4(+) and CD8(+)) and TCR gamma delta (+) T cells with regard to IFN-gamma production. However, crosslinking of CD81 molecules on TCR gamma delta (+) T cells, in the absence of anti-CD3 stimulation, resulted in cytokine production and enhanced IL-a-induced proliferation, demonstrating that physical association with CD4 or CD8 is not necessary for CD81 signaling. In contrast, crosslinking of CD81 on TCR alpha beta (+) T cells, in the absence of anti-CD3 stimulation, failed to activate these T cells. These results suggest that CD81 signaling may be mediated via a different mechanism(s) in TCR gamma delta (+) versus TCR alpha beta (+) T cells. (C) 2001 Academic Press.
引用
收藏
页码:19 / 27
页数:9
相关论文
共 47 条
[1]  
ANGELISOVA P, 1994, IMMUNOGENETICS, V39, P249
[2]   ADHESION AND COSTIMULATION OF PROLIFERATIVE RESPONSES OF HUMAN GAMMA-DELTA T-CELLS BY INTERACTION OF VLA-4 AND VLA-5 WITH FIBRONECTIN [J].
AVDALOVIC, M ;
FONG, D ;
FORMBY, B .
IMMUNOLOGY LETTERS, 1993, 35 (02) :101-108
[3]   ENGAGING CD19 OR TARGET OF AN ANTIPROLIFERATIVE ANTIBODY-1 ON HUMAN B-LYMPHOCYTES INDUCES BINDING OF B-CELLS TO THE INTERFOLLICULAR STROMA OF HUMAN TONSILS VIA INTEGRIN-ALPHA-4/BETA-1 AND FIBRONECTIN [J].
BEHR, S ;
SCHRIEVER, F .
JOURNAL OF EXPERIMENTAL MEDICINE, 1995, 182 (05) :1191-1199
[4]   A novel link between integrins, transmembrane-4 superfamily proteins (CD63 and CD81), and phosphatidylinositol 4-kinase [J].
Berditchevski, F ;
Tolias, KF ;
Wong, K ;
Carpenter, CL ;
Hemler, ME .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (05) :2595-2598
[5]   Characterization of novel complexes on the cell surface between integrins and proteins with 4 transmembrane domains (TM4 proteins) [J].
Berditchevski, F ;
Zutter, MM ;
Hemler, ME .
MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (02) :193-207
[6]   SPECIFIC ASSOCIATION OF CD63 WITH THE VLA-3 AND VLA-6 INTEGRINS [J].
BERDITCHEVSKI, F ;
BAZZONI, G ;
HEMLER, ME .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (30) :17784-17790
[7]  
BRADBURY LE, 1992, J IMMUNOL, V149, P2841
[8]  
BROOKS EG, 1990, J IMMUNOL, V144, P4507
[9]   CD19 - LOWERING THE THRESHOLD FOR ANTIGEN RECEPTOR STIMULATION OF LYMPHOCYTES-B [J].
CARTER, RH ;
FEARON, DT .
SCIENCE, 1992, 256 (5053) :105-107
[10]   INTEGRINS AND SIGNAL-TRANSDUCTION PATHWAYS - THE ROAD TAKEN [J].
CLARK, EA ;
BRUGGE, JS .
SCIENCE, 1995, 268 (5208) :233-239