Chemiluminescent optical fiber immunosensor for the detection of anti-West Nile virus IgG

被引：27

作者：

Herrmann, S

Leshem, B

Landes, S

Rager-Zisman, B

Marks, RS ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Inst Appl Biosci, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Dept Microbiol & Immunol, IL-84105 Beer Sheva, Israel

[4] Ben Gurion Univ Negev, Fac Hlth Sci, Canc Res Ctr, IL-84105 Beer Sheva, Israel

来源：

TALANTA | 2005年 / 66卷 / 01期

关键词：

optical fiber immunosensor; ELISA; West Nile virus; chemiluminescence;

D O I：

10.1016/j.talanta.2004.10.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An ELISA-based optical fiber methodology developed for the detection of anti-West Nile vir-us IgG antibodies in serum was compared to standard colorimetric and chemiluminescent ELISA based on microtiter plates. Colorimetric ELISA was the least sensitive, especially at high titer dilutions. The fiber-optic immunosensor based on the same ELISA immunological rationale was the most sensitive technique. (c) 2004 Elsevier B.V. All rights reserved.

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页码：6 / 14

页数：9

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