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The properties of the mitochondrial megachannel in mitoplasts from human colon carcinoma cells are not influenced by Bax

被引：23
作者：
Campello, S
De Marchi, U
Szabò, I
Tombola, F
Martinou, JC
Zoratti, M
机构：
[1] Univ Padua, Dept Biomed Sci, CNR, Inst Neurosci,Biomembranes Sect, I-35121 Padua, Italy
[2] Univ Padua, Dept Biol, Padua, Italy
[3] Univ Geneva, Dept Cell Biol, Geneva, Switzerland
来源：
FEBS LETTERS | 2005年 / 579卷 / 17期
关键词：
permeability transition pore; mitochondria; apoptosis; Bax; patch-clamp;
D O I：
10.1016/j.febslet.2005.05.055
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper explores the relationship between Bax and the mitochondrial permeability transition pore (PTP). Isolated human colon tumor (HCT116) Bax(-) mitochondria exposed to recombinant Bax exhibited a slow, cyclosporin A-sensitive swelling, but only at [Bax] > 200 nM. The amount of Bax incorporated was much higher than that found in organelles isolated from HCT116 Bax(+) staurosporine- or etoposide-treated apoptotic cells, casting doubts on the significance of the putative PT induction for apoptosis. Bax did not influence the electrophysiological properties of an approximately 1 nS channel ascribed to the Ca2+-dependent mitochondrial permeability transition pore. These observations indicate that the PTP is independent of Bax. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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收藏
页码：3695 / 3700
页数：6
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