Cloning and sequencing of the group 6 allergen of Dermatophagoides pteronyssinus

被引：25

作者：

Bennett, BJ ^{[1
]}

Thomas, WR ^{[1
]}

机构：

[1] TVW,TELETHON INST CHILD HLTH RES,PERTH,WA 6872,AUSTRALIA

来源：

CLINICAL AND EXPERIMENTAL ALLERGY | 1996年 / 26卷 / 10期

关键词：

Der p 6; house dust mite; Dermatophagoides pteronyssinus; cDNA; cDNA sequence; chymotrypsin;

D O I：

10.1111/j.1365-2222.1996.tb00501.x

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background The allergen Der p 6 from Dermatophagoides pteronysinus has been described by substrate affinity as mite chymotrypsin. Objective The aim of this paper was to describe a cDNA clone encoding the allergen. Methods cDNA was cloned from a lambda library using oligonucleotides published for Der p 6. Results The clone P6.1.1 had the N-terminal amino acid residues as reported for Der p 6, and at position 189 the Ser was conserved in chymotrypsin for substrate specificity as well as the catalytic triad of His57, Asp102 and Ser195 for serine proteases. The estimated Mr was 24.9 K and it was 37% identical to the trypsin allergen Der p3. Conclusion cDNA encoding Der p 6 has been described.

引用

页码：1150 / 1154

页数：5

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