Protein Lipidation Catalyzed by Microbial Transglutaminase

被引：34

作者：

Abe, Hiroki ^{[1
]}

Goto, Masahiro ^{[1
,2
]}

Kamiya, Noriho ^{[1
,2
]}

机构：

[1] Kyushu Univ, Dept Appl Chem, Grad Sch Engn, Nishi Ku, Fukuoka 8190395, Japan

[2] Kyushu Univ, Ctr Future Chem, Fukuoka 812, Japan

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2011年 / 17卷 / 50期

基金：

日本学术振兴会;

关键词：

enzymes; lipid modifications; peptides; protein engineering; transglutaminase; RECOMBINANT; LIGATION; BILAYERS; PROBE;

D O I：

10.1002/chem.201102121

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fattening up: Artificially constructed lipid-protein conjugates can be used to control protein activity and localization at either biological or artificial membrane interfaces. Here, we report a facile technique for the lipid modification of proteins catalyzed by microbial transglutaminase (MTG). MTG-mediated protein lipidation proceeded efficiently without perturbing protein functionality (see figure). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：14004 / 14008

页数：5

共 32 条

[1] Enzymatic single-step preparation of multifunctional proteins [J].

Abe, Hiroki ;

Goto, Masahiro ;

Kamiya, Noriho .

CHEMICAL COMMUNICATIONS, 2010, 46 (38) :7160-7162

[2] PURIFICATION AND CHARACTERISTICS OF A NOVEL TRANSGLUTAMINASE DERIVED FROM MICROORGANISMS [J].

ANDO, H ;

ADACHI, M ;

UMEDA, K ;

MATSUURA, A ;

NONAKA, M ;

UCHIO, R ;

TANAKA, H ;

MOTOKI, M .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (10) :2613-2617

[3] Lipid Modification of Proteins through Sortase-Catalyzed Transpeptidation [J].

Antos, John M. ;

Miller, Gwenn M. ;

Grotenbreg, Gijsbert M. ;

Ploegh, Hidde L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (48) :16338-16343

[4] Bioorganic synthesis of lipid-modified proteins for the study of signal transduction [J].

Bader, B ;

Kuhn, K ;

Owen, DJ ;

Waldmann, H ;

Wittinghofer, A ;

Kuhlmann, J .

NATURE, 2000, 403 (6766) :223-226

[5] Selective labeling of proteins by using protein farnesyltransferase [J].

Duckworth, Benjamin P. ;

Zhang, Zhiyuan ;

Hosokawa, Ayako ;

Distefano, Mark D. .

CHEMBIOCHEM, 2007, 8 (01) :98-105

[6] Characterization of recombinant, membrane-attached full-length prion protein [J].

Eberl, H ;

Tittmann, P ;

Glockshuber, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (24) :25058-25065

[7] REQUIREMENT OF PROTEIN ASSOCIATION WITH MEMBRANES FOR PHOSPHORYLATION BY PROTEIN-KINASE-C [J].

EDASHIGE, K ;

UTSUMI, T ;

SATO, EF ;

IDE, A ;

KASAI, M ;

UTSUMI, K .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1992, 296 (01) :296-301

[8] Protein N-terminal methionine excision [J].

Giglione, C ;

Boularot, A ;

Meinnel, T .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2004, 61 (12) :1455-1474

[9] Synthesis of lipidated green fluorescent protein and its incorporation in supported lipid bilayers [J].

Grogan, MJ ;

Kaizuka, Y ;

Conrad, RM ;

Groves, JT ;

Bertozzi, CR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (41) :14383-14387

[10] Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry [J].

Heal, William P. ;

Wickramasinghe, Sasala R. ;

Bowyer, Paul W. ;

Holder, Anthony A. ;

Smith, Deborah F. ;

Leatherbarrow, Robin J. ;

Tate, Edward W. .

CHEMICAL COMMUNICATIONS, 2008, (04) :480-482

← 1 2 3 4 →