Characterization of four terpene synthase cDNAs from methyl jasmonate-induced Douglas-fir, Pseudotsuga menziesii

被引：62

作者：

Huber, DPW

Philippe, RN

Godard, KA

Sturrock, RN

Bohlmann, J

机构：

[1] Univ British Columbia, Michael Smith Labs, Vancouver, BC V6T 1Z4, Canada

[2] Univ British Columbia, Dept Bot, Vancouver, BC V6T 1Z4, Canada

[3] Univ British Columbia, Dept Forest Sci, Vancouver, BC V6T 1Z4, Canada

[4] Canadian Forest Serv, Pacific Forestry Ctr, Victoria, BC V8Z 1M5, Canada

来源：

PHYTOCHEMISTRY | 2005年 / 66卷 / 12期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Pseudotsuga menziesii; Pinaceae; conifer defense; oleoresin; terpenoid synthases;

D O I：

10.1016/j.phytochem.2005.04.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous terpenoid compounds are present in copious amounts in the oleoresin produced by conifers, especially following exposure to insect or fungal pests. CDNA clones for many terpene synthases responsible for the biosynthesis of these defense compounds have been recovered from several conifer species. Here, the use of three terpene synthase sequences as heterologous probes for the discovery of related terpene synthase genes in Douglas-fir, Pseudotsuga menziesii (Mirbel) Franco (Pinaceae), is reported. Four full-length terpene synthase cDNAs were recovered from a methyl jasmonate-induced Douglas-fir bark and shoot cDNA library. These clones encode two multi-product monoterpene synthases [a (-)-alpha-pinene/(-)-camphene synthase and a terpinolene synthase] and two single-product sesquiterpene synthases [an (E)-beta-farnesene synthase and a (E)-gamma-bisabolene synthase]. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1427 / 1439

页数：13

共 35 条

[1]

Adams R., 2001, Identification of essential oil components by gas chromatography/quadrupole mass spectrometry

[2] Mechanisms of resistance in conifers against shoot infesting insects -: The case of the white pine weevil Pissodes strobi (Peck) -: (Coleoptera: Curculionidae) [J].