Altered expression of sodium transporters in ischemic penumbra after focal cerebral ischemia in rats

被引:30
作者
Jung, Yong-Wook
Choi, In-Jang
Kwon, Tae-Hwan
机构
[1] Kyungpook Natl Univ, Sch Med, Dept Biochem & Cell Biol, Taegu 700422, South Korea
[2] Keimyung Univ, Coll Med, Dept Anat, Taegu 700712, South Korea
[3] Dongguk Univ, Coll Med, Dept Anat, Kyungju 700422, South Korea
关键词
brain ischemia; brain edema; intracellular acidosis; ischemic penumbra; sodium transporters; targeted proteomics;
D O I
10.1016/j.neures.2007.06.1470
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
This study was aimed to examine whether the changes of protein expression of sodium transporters in the ischemic penumbra are associated with the pathogenesis of ischemia-induced brain edema and/or brain cell injury. An experimental model of cerebral ischemia was made by permanent middle cerebral artery occlusion (pMCAO) in rats and the changes of protein expression of sodium transporters in the ischemic penumbra were examined by immunoblotting. Extensive infarction was observed in the frontal and parietal cortical and subcortical areas at 3 and 6 h after pMCAO. Immunoblotting analyses revealed significantly increased expressions of electrogenic NBC (241 +/- 11 % at 3 h and 154 +/- 9% at 6 h, P < 0.05) and NHEI (144 +/- 3% at 3 It and 170 +/- 9% at 6 h, P < 0.05), compared with sham-operated controls. In contrast, Na-K-ATPase expression (78 +/- 6% at 3 It and 85 +/- 3% at 6 h, P < 0.05) was significantly decreased. The expression of NCX1 was unchanged at 3 h, but was significantly increased at 6 h (141 3%, P < 0.05). In addition, the expressions of neuronal (NeuN) and astroglial cell (GFAP) proteins were decreased, whereas the expression of oligodendrocyte protein (CNPase) was unchanged. Taken together, the selectively increased expressions of NHEI, electrogenic NBC, and NCX1 and decreased expression of Na-K-ATPase in the ischemic penumbra are likely to contribute to the secondary brain cell damages presumably through intracellular Na+ accumulation, cell swelling, and intracellular Ca2+ overload. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All fights reserved.
引用
收藏
页码:152 / 159
页数:8
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