Hydrogen peroxide determination in pharmaceutical formulations and cosmetics using a new catalase biosensor

被引:81
作者
Campanella, L [1 ]
Roversi, R [1 ]
Sammartino, MP [1 ]
Tomassetti, M [1 ]
机构
[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
关键词
hydrogen peroxide biosensor; analysis; cosmetics; pharmaceutical formulations;
D O I
10.1016/S0731-7085(98)00155-1
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The possibility of evaluating the content of hydrogen peroxide in several authentic matrices, such as cosmetic and pharmaceutical formulations, was studied. A new catalase biosensor fabricated using an amperometric gas-diffusion oxygen sensor as electrochemical transducer and the catalase enzyme immobilized in kappa-carrageenan gel and capable of operating in both aqueous and non aqueous solvents was developed and tested for this purpose. Creams, emulsions and disinfectant solutions were analysed. To this end, a preliminary check was needed to establish the best conditions to analyse these matrices; the choice of solvent was one of the most important points studied. The solvents considered included dioxane, water-dioxane mixtures, water saturated chloroform and aqueous solutions. The different solubility properties of the matrices analysed were taken into account. (C) 1997 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:105 / 116
页数:12
相关论文
共 21 条
  • [1] BOTTARI E, 1978, ANAL CHIM QUANTITATI, V6, P267
  • [2] TYROSINASE BIOSENSOR RESPONSE AS A FUNCTION OF PHYSICAL-PROPERTIES OF ORGANIC-SOLVENTS
    CAMPANELLA, L
    FORTUNEY, A
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. TALANTA, 1994, 41 (08) : 1397 - 1404
  • [3] THE EFFECT OF ORGANIC-SOLVENT PROPERTIES ON THE RESPONSE OF A TYROSINASE ENZYME SENSOR
    CAMPANELLA, L
    FAVERO, G
    SAMMARTINO, MP
    TOMASSETTI, M
    [J]. TALANTA, 1994, 41 (06) : 1015 - 1023
  • [4] Campanella L, 1996, ANALUSIS, V24, P288
  • [5] The effect of organic solvent properties on a catalase enzyme sensor for monitoring hydrogen peroxide in nonaqueous solutions
    Campanella, L
    Martini, U
    Sammartino, MP
    Tomassetti, M
    [J]. ELECTROANALYSIS, 1996, 8 (12) : 1150 - 1154
  • [6] CAMPANELLA L, 1990, BIOINSTRUMENTATION R, P1369
  • [7] CAMPANELLA L, 1990, SEL ELECTRODE REV, V11, P69
  • [8] CONSTRUCTION OF A TYROSINASE-BASED BIOSENSOR IN PURE ORGANIC-PHASE
    DENG, Q
    DONG, SJ
    [J]. ANALYTICAL CHEMISTRY, 1995, 67 (08) : 1357 - 1360
  • [9] GUILBAULT GG, 1970, ENZYMATIC METHODS AN
  • [10] THE DETERMINATION OF P-CRESOL IN CHLOROFORM WITH AN ENZYME ELECTRODE USED IN THE ORGANIC-PHASE
    HALL, GF
    BEST, DJ
    TURNER, APF
    [J]. ANALYTICA CHIMICA ACTA, 1988, 213 (1-2) : 113 - 119