Genetic heterogeneity of bovine viral diarrhoea viruses from Spain

被引：32

作者：

Arias, P

Orlich, M

Prieto, M

Rosales, SC

Thiel, HJ

Alvarez, M

Becher, P

机构：

[1] Univ Giessen, Inst Virol, Fac Med Vet, D-35392 Giessen, Germany

[2] Univ Leon, Fac Vet, Dept Patol, E-24071 Leon, Spain

来源：

VETERINARY MICROBIOLOGY | 2003年 / 96卷 / 04期

关键词：

bovine viral diarrhoea virus; genotyping; cattle; viruses; diagnosis;

D O I：

10.1016/j.vetmic.2003.09.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Forty-four bovine viral diarrhoea virus (BVDV) isolates collected from the north of Spain between 1989 and 2000 were characterised at the molecular level. For 24 of these isolates the entire N-pro gene sequence was determined. Phylogenetic analysis revealed that 21 isolates belong to subgroup BVDV-1b, while two BVDV-1c isolates and one BVDV-1h isolate were found. Furthermore, 20 additional virus isolates were analysed by differential reverse transcription-polymerase chain reaction (RT-PCR) and classified as BVDV-1. The results from our study demonstrate that BVDV-1b is the most prevalent subgroup present in bovine dairy herds of Spain, while there is no evidence for the presence of BVDV-2. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：327 / 336

页数：10

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