Optimization of fermentation conditions for the production of ethanol from sago starch by co-immobilized amyloglucosidase and cells of Zymomonas mobilis using response surface methodology

被引：86

作者：

Bandaru, VVR

Somalanka, SR

Mendu, DR

Madicherla, NR

Chityala, A

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21287 USA

[2] Andhra Univ, Coll Engn, Dept Chem Engn, Ctr Biotechnol, Visakhapatnam 550003, Andhra Pradesh, India

[3] Johns Hopkins Univ, Sch Med, Dept Pediat, Div Infect Dis, Baltimore, MD 21205 USA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 38卷 / 1-2期

关键词：

co-immobilization; amyloglucosidase; sago starch; ethanol; response surface methodology;

D O I：

10.1016/j.enzmictec.2005.06.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Statistical experimental design was used to optimize the conditions of simultaneous saccharification and fermentation (SSF), viz. temperature, pH and time of fermentation of ethanol from sago starch with co-immobilized amyloglucosidase (AMG) and Zymomonas mobilis MTCC 92 by submerged fermentation. Maximum ethanol concentration of 55.3 all was obtained using a starch concentration of 150 g/l. The optimum conditions were found to be a temperature of 32.4 degrees C, pH of 4.93 and time of fermentation of 17.24 h. Thus, by using SSF process with co-immobilized AMG and Z mobilis cells MTCC 92, the central composite design (CCD) was found to be the most favourable strategy investigated with respect to ethanol production and enzyme recovery. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：209 / 214

页数：6

共 20 条

[1] Optimizing medium constituents and fermentation conditions for citric acid production from palmyra jaggery using response surface method [J].