Use of a Cry1Ac-resistant line of Helicoverpa armigera (Lepidoptera: Noctuidae) to detect novel insecticidal toxin genes in Bacillus thuringiensis

被引：6

作者：

Beard, Cheryl E. ^{[1
]}

Court, Leon ^{[1
]}

Mourant, Roslyn G. ^{[1
]}

James, Bill ^{[1
]}

Van Rie, Jeroen ^{[2
]}

Masson, Luke ^{[3
]}

Akhurst, Raymond J. ^{[1
]}

机构：

[1] CSIRO Entomol, Canberra, ACT 2601, Australia

[2] Bayer BioSci NV, B-9052 Ghent, Belgium

[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

CURRENT MICROBIOLOGY | 2008年 / 57卷 / 03期

关键词：

D O I：

10.1007/s00284-008-9098-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac (line ISOC) was used to screen Cry1Ac-expressing strains of B. thuringiensis for additional toxins with activity against H. armigera. Using this approach, a number of Cry1Ac-producing strains with significant toxicity toward Cry1Ac-resistant H. armigera were identified. When the insecticidal protein complement of one of these strains, C81, was examined in detail, a novel cry2 gene (cry2Af1) was detected.

引用

页码：175 / 180

页数：6

共 25 条

[1] Resistance to the Cry1Ac δ-endotoxin of Bacillus thuringiensis in the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) [J].