Metagenomics for studying unculturable microorganisms: cutting the Gordian knot

被引：272

作者：

Schloss, PD ^{[1
]}

Handelsman, J ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA

来源：

GENOME BIOLOGY | 2005年 / 6卷 / 08期

关键词：

D O I：

10.1186/gb-2005-6-8-229

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

More than 99% of prokaryotes in the environment cannot be cultured in the laboratory, a phenomenon that limits our understanding of microbial physiology, genetics, and community ecology. One way around this problem is metagenomics, the culture-independent cloning and analysis of microbial DNA extracted directly from an environmental sample. Recent advances in shotgun sequencing and computational methods for genome assembly have advanced the field of metagenomics to provide glimpses into the life of uncultured microorganisms.

引用

页数：4

共 20 条

[1] Community genomics in microbial ecology and evolution [J].

Allen, EE ;

Banfield, JF .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (06) :489-498

[2] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].

AMANN, RI ;

BINDER, BJ ;

OLSON, RJ ;

CHISHOLM, SW ;

DEVEREUX, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925

[3]

[Anonymous], KEGG: Kyoto Encyclopedia of Genes and genomes

[4] Microbial community genomics in the ocean [J].

DeLong, EE .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (06) :459-469

[5] Metagenomics or megagenomics? [J].

Handelsman, J .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (06) :457-458

[6] Chitinase gene sequences retrieved from diverse aquatic habitats reveal environment-specific distributions [J].

LeCleir, GR ;

Buchan, A ;

Hollibaugh, JT .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (12) :6977-6983

[7] Alternative oxidase and plastoquinol terminal oxidase in marine prokaryotes of the Sargasso Sea [J].

McDonald, AE ;

Vanlerberghe, GC .

GENE, 2005, 349 :15-24

[8]

Miller DN, 1999, APPL ENVIRON MICROB, V65, P4715

[9] Genomic sequencing of Pleistocene cave bears [J].

Noonan, JP ;

Hofreiter, M ;

Smith, D ;

Priest, JR ;

Rohland, N ;

Rabeder, G ;

Krause, J ;

Detter, JC ;

Pääbo, S ;

Rubin, EM .

SCIENCE, 2005, 309 (5734) :597-600

[10] Community proteomics of a natural microbial biofilm [J].

Ram, RJ ;

VerBerkmoes, NC ;

Thelen, MP ;

Tyson, GW ;

Baker, BJ ;

Blake, RC ;

Shah, M ;

Hettich, RL ;

Banfield, JF .

SCIENCE, 2005, 308 (5730) :1915-1920

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