Phase transitions in biogenic amorphous calcium carbonate

被引：258

作者：

Gong, Yutao U. T. ^{[1
]}

Killian, Christopher E. ^{[1
,2
]}

Olson, Ian C. ^{[1
]}

Appathurai, Narayana P. ^{[3
]}

Amasino, Audra L. ^{[1
]}

Martin, Michael C. ^{[4
]}

Holt, Liam J. ^{[2
]}

Wilt, Fred H. ^{[2
]}

Gilbert, P. U. P. A. ^{[1
,5
]}

机构：

[1] Univ Wisconsin, Dept Phys, Madison, WI 53706 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA

[4] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Adv Light Source, Berkeley, CA 94720 USA

[5] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2012年 / 109卷 / 16期

基金：

美国国家科学基金会;

关键词：

calcite; synchrotron; larva; echinoderm; echinodermata; SEA-URCHIN EMBRYO; ORGANIC-MINERAL INTERFACE; SPICULE MATRIX PROTEIN; PRECURSOR PHASE; BIOMINERALIZATION; TRANSFORMATION; COMPONENT; GENE; KEY;

D O I：

10.1073/pnas.1118085109

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Crystalline biominerals do not resemble faceted crystals. Current explanations for this property involve formation via amorphous phases. Using X-ray absorption near-edge structure (XANES) spectroscopy and photoelectron emission microscopy (PEEM), here we examine forming spicules in embryos of Strongylocentrotus purpuratus sea urchins, and observe a sequence of three mineral phases: hydrated amorphous calcium carbonate (ACC center dot H2O) -> dehydrated amorphous calcium carbonate (ACC) -> calcite. Unexpectedly, we find ACC center dot H2O-rich nanoparticles that persist after the surrounding mineral has dehydrated and crystallized. Protein matrix components occluded within the mineral must inhibit ACC center dot H2O dehydration. We devised an in vitro, also using XANES-PEEM, assay to identify spicule proteins that may play a role in stabilizing various mineral phases, and found that the most abundant occluded matrix protein in the sea urchin spicules, SM50, stabilizes ACC center dot H2O in vitro.

引用

页码：6088 / 6093

页数：6

共 59 条

[1]

Stabilization of amorphous calcium carbonate by specialized macromolecules in biological and synthetic precipitates [J].