K+ATP channel opening prevents succinate-dependent H2O2 generation by plant mitochondria

被引:17
作者
Casolo, V [1 ]
Braidot, E [1 ]
Chiandussi, E [1 ]
Vianello, A [1 ]
Macrì, F [1 ]
机构
[1] Univ Udine, Dept Biol & Agroind Econ, Plant Biol Sect, I-33100 Udine, Italy
关键词
D O I
10.1034/j.1399-3054.2003.00109.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The role of a recently identified K-ATP(+) channel in preventing H2O2 formation was examined in isolated pea stem mitochondria. The succinate-dependent H2O2 formation was progressively inhibited, when mitochondria were resuspended in media containing increasing concentration of KCl (from 0.05 to 0.15 M). This inhibition was linked to a partial dissipation of the transmembrane electrical potential (DeltaPsi) induced by KCl. Conversely, the malate plus glutamate-dependent H2O2 formation was not influenced. The succinate-sustained H2O2 generation was also unaffected by nigericin (a H+/K+ exchanger), but completely prevented by valinomycin (a K+ ionophore). In addition, cyclosporin A (a K-ATP(+) channel opener) inhibited this H2O2 formation, while ATP (an inhibitor of the channel opening) slightly increased it. The inhibitory effect of ATP was strongly stimulated in the presence of atractylate (an inhibitor of the adenine nucleotide translocase), thus suggesting that the receptor for ATP on the K+ channel faces the intermembrane space. Finally, the succinate-dependent H2O2 formation was partially prevented by phenylarsine oxide (a thiol oxidant).
引用
收藏
页码:313 / 318
页数:6
相关论文
共 27 条
[1]  
Alscher RG, 1997, PHYSIOL PLANTARUM, V100, P224, DOI 10.1034/j.1399-3054.1997.1000203.x
[2]   Occurrence and characteristics of the mitochondrial permeability transition in plants [J].
Arpagaus, S ;
Rawyler, A ;
Braendle, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (03) :1780-1787
[3]   RECENT PROGRESS ON REGULATION OF THE MITOCHONDRIAL PERMEABILITY TRANSITION PORE - A CYCLOSPORINE-SENSITIVE PORE IN THE INNER MITOCHONDRIAL-MEMBRANE [J].
BERNARDI, P ;
BROEKEMEIER, KM ;
PFEIFFER, DR .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1994, 26 (05) :509-517
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   Hydrogen peroxide generation by higher plant mitochondria oxidizing complex I or complex II substrates [J].
Braidot, E ;
Petrussa, E ;
Vianello, A ;
Macrì, F .
FEBS LETTERS, 1999, 451 (03) :347-350
[6]   Pyrophosphate import and synthesis by plant mitochondria [J].
Casolo, V ;
Micolini, S ;
Macrì, F ;
Vianello, A .
PHYSIOLOGIA PLANTARUM, 2002, 114 (04) :516-523
[7]   The role of mild uncoupling and non-coupled respiration in the regulation of hydrogen peroxide generation by plant mitochondria [J].
Casolo, V ;
Braidot, E ;
Chiandussi, E ;
Macri, F ;
Vianello, A .
FEBS LETTERS, 2000, 474 (01) :53-57
[8]   Modulation of a plant mitochondrial K+ATP channel and its involvement in cytochrome c release [J].
Chiandussi, E ;
Petrussa, E ;
Macrì, F ;
Vianello, A .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2002, 34 (03) :177-184
[9]   Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells [J].
Corpas, FJ ;
Barroso, JB ;
del Río, LA .
TRENDS IN PLANT SCIENCE, 2001, 6 (04) :145-150
[10]   Ca2+ induces a cyclosporin A-insensitive permeability transition pore in isolated potato tuber mitochondria mediated by reactive oxygen species [J].
Fortes, F ;
Castilho, RF ;
Catisti, R ;
Carnieri, EGS ;
Vercesi, AE .
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 2001, 33 (01) :43-51