Environmental isolates of Aeromonas spp. harboring the cagA-like gene of Helicobacter pylori

被引：9

作者：

Datta, S

Khan, A

Nandy, RK

Rehman, M

Sinha, S

Chattopadhyay, S

Das, SC

Nair, GB

机构：

[1] Int Ctr Diarrhoeal Dis Res, Div Sci Lab, ICDDR B, Dhaka 1212, Bangladesh

[2] Natl Inst Cholera & Enter Dis, Kolkata 700010, W Bengal, India

[3] ICMR Virus Unit, Kolkata 700010, W Bengal, India

[4] Indian Vet Res Inst, Kolkata 700037, W Bengal, India

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 07期

关键词：

D O I：

10.1128/AEM.69.7.4291-4295.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.

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页码：4291 / 4295

页数：5

相关论文

共 16 条

[1] The Helicobacter pylori genome sequence:: genetic factors for long life in the gastric mucosa [J].

Berg, DE ;

Hoffman, PS ;

Appelmelk, BJ ;

Kusters, JG .

TRENDS IN MICROBIOLOGY, 1997, 5 (12) :468-474

[2] cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors [J].

Censini, S ;

Lange, C ;

Xiang, ZY ;

Crabtree, JE ;

Ghiara, P ;

Borodovsky, M ;

Rappuoli, R ;

Covacci, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (25) :14648-14653

[3] Detection of Aeromonas hydrophila in a drinking- water distribution system:: a field and pilot study [J].

Chauret, C ;

Volk, C ;

Creason, R ;

Jarosh, J ;

Robinson, J ;

Warnes, C .

CANADIAN JOURNAL OF MICROBIOLOGY, 2001, 47 (08) :782-786

[4] Mechanisms causing rapid and parallel losses of ribose catabolism in evolving populations of Escherichia coli B [J].

Cooper, VS ;

Schneider, D ;

Blot, M ;

Lenski, RE .

JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2834-2841

[5] Prevalence, species differentiation, haemolytic activity, and antibiotic susceptibility of aeromonads in untreated well water [J].

Ghenghesh, KS ;

El-Ghodban, A ;

Dkakni, R ;

Abeid, S ;

Altomi, A ;

Tarhuni, A ;

Marialigeti, K .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2001, 96 (02) :169-173

[6] Genotyping of Helicobacter pylori isolates by sequencing of PCR products and comparison with the RAPD technique [J].

Kansau, I ;

Raymond, J ;

Bingen, E ;

Courcoux, P ;

Kalach, N ;

Bergeret, M ;

Braimi, N ;

Dupont, C ;

Labigne, A .

RESEARCH IN MICROBIOLOGY, 1996, 147 (08) :661-669

[7] Novel sequence organization and insertion specificity of IS605 and IS606:: chimaeric transposable elements of Helicobacter pylori [J].

Kersulyte, D ;

Akopyants, NS ;

Clifton, SW ;

Roe, BA ;

Berg, DE .

GENE, 1998, 223 (1-2) :175-186

[8] Transposable element ISHp608 of Helicobacter pylori:: Nonrandom geographic distribution, functional organization, and insertion specificity [J].

Kersulyte, D ;

Velapatiño, B ;

Dailide, G ;

Mukhopadhyay, AK ;

Ito, Y ;

Cahuayme, L ;

Parkinson, AJ ;

Gilman, RH ;

Berg, DE .

JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :992-1002

[9] Functional organization and insertion specificity of IS607, a chimeric element of Helicobacter pylori [J].

Kersulyte, D ;

Mukhopadhyay, AK ;

Shirai, M ;

Nakazawa, T ;

Berg, DE .

JOURNAL OF BACTERIOLOGY, 2000, 182 (19) :5300-5308

[10] Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater [J].

Lu, YZ ;

Redlinger, TE ;

Avitia, R ;

Galindo, A ;

Goodman, K .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (03) :1436-1439