Reverse transcription-detection of immobilized, amplified product in a one-phase system (RT-DIAPOPS) for the detection of potato virus Y

被引：2

作者：

Nicolaisen, M ^{[1
]}

Rasmussen, HN

Husted, K

Nielsen, SL

机构：

[1] Danish Inst Agr Sci, Dept Plant Protect, Res Ctr Flakkebjerg, DK-4200 Slagelse, Denmark

[2] NUNC AS, DK-4000 Roskilde, Denmark

来源：

PLANT PATHOLOGY | 2001年 / 50卷 / 01期

关键词：

DIAPOPS; PCR; potato virus Y;

D O I：

10.1046/j.1365-3059.2001.00527.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

An RT-DIAPOPS (reverse transcription-detection of immobilized, amplified product in a one-phase system) assay was developed for the detection of potato virus Y (PVY). The sensitivity of the assay was 0.5 pg when virions were added directly to the RT reaction. The assay was capable of detecting all isolates investigated, representing several taxonomic groups of PVY. The RT-DIAPOPS offers an attractive alternative to RT-polymerase chain reaction (PCR) as it does not depend on gel electrophoresis to detect virus-specific PCR products. Finally, as amplicons are covalently attached to a solid phase, it minimizes the risk of contamination.

引用

页码：124 / 129

页数：6

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