Whole-mount in situ hybridization to mouse embryos

被引:44
作者
Correia, KM [1 ]
Conlon, RA [1 ]
机构
[1] Case Western Reserve Univ, Univ Hosp, Dept Genet, Cleveland, OH 44106 USA
关键词
D O I
10.1006/meth.2000.1145
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The mouse is well-established as the major animal model for the study of mammalian development. Rapid progress in large-scale cDNA and also genomic sequencing projects is identifying new mouse genes at an unprecedented rate. As a first step toward understanding the function of these novel genes, it is important to determine their developmental expression pattern. Here we provide a reliable, sensitive method for wholemount in situ hybridization using the mouse embryo. (C) 2001 Academic Press.
引用
收藏
页码:335 / 338
页数:4
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