Neuronal birthdate-specific gene transfer with adenoviral vectors

被引:33
作者
Hashimoto, M
Mikoshiba, K
机构
[1] RIKEN, Brain Sci Inst, Dev Neurobiol Lab, Wako, Saitama 3510198, Japan
[2] Univ Tokyo, Inst Med Sci, Dept Mol Neurobiol, Tokyo 1088639, Japan
关键词
neuronal birthdate; gene transfer; cerebral cortex; adenoviral vector; development; laminar structure;
D O I
10.1523/JNEUROSCI.2529-03.2004
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The multilayered structure of the cerebral cortex has been studied in detail. Early-born neurons migrate into the inner layer and late-born neurons migrate into more superficial layers, thus establishing an inside-out gradient. The progenitor cells appear to acquire layer-specific properties at the time of neuronal birth; however, the molecular mechanisms of cell-fate acquisition are still unclear, because it has been difficult to identify a cohort of birthdate-related progenitor cells. Using replication-defective adenoviral vectors, we successfully performed "pulse gene transfer" into progenitor cells in a neuronal birthdate-specific manner. When adenoviral vectors were injected into the midbrain ventricle of mouse embryos between embryonic day 10.5 (E10.5) and E14.5, the adenoviral vectors introduced a foreign gene into a specific cohort of birthdate-related progenitor cells. The virally infected cohorts developed normally into cortical neurons and formed the canonical cortical layers in an inside-out manner. This technique allows us to distinguish a cohort of birthdate-related progenitor cells from other progenitor cells with different birthdates and to introduce a foreign gene into specific subsets of cortical layers by performing adenoviral injection at specific times. This adenovirus- meditated gene transfer technique will enable us to examine the properties of each subset of progenitor cells that share the same neuronal birthdate.
引用
收藏
页码:286 / 296
页数:11
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