A biosensor for estrogenic substances using the quartz crystal microbalance

被引：23

作者：

Carmon, KS

Baltus, RE

Luck, LA ^{[1
]}

机构：

[1] Clarkson Univ, Dept Chem, Potsdam, NY 13699 USA

[2] Clarkson Univ, Dept Chem Engn, Potsdam, NY 13699 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 345卷 / 02期

关键词：

estrogen receptor; biosensor; quartz crystal microbalance; estrogens; xenoestrogens; surface chemistry;

D O I：

10.1016/j.ab.2005.07.018

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This article describes a biosensor that detects estrogenic substances using a quartz crystal microbalance with a genetically engineered construct of the hormone-binding domain of the alpha-estrogen receptor. The receptor was immobilized to a piezoelectric quartz crystal via a single exposed cysteine, forming a uniform orientation on the crystal surface. Our results illustrate that this sensor responds to a variety of ligands that are known to bind to the estrogen receptor. No response was observed for nonbinding substances such as testosterone and progesterone. The sensitive response of this biosensor to estrogenic substances results from changes in the structural rigidity of the immobilized receptor that occurs with ligand binding. Agonist and antagonist show different responses. (C) 2005 Elsevier Inc. All rights reserved.

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收藏

页码：277 / 283

页数：7

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