Cloning and sequencing of a gene encoding 16S ribosomal RNA from a novel hyperthermophilic archaebacterium NC12

被引：10

作者：

Aoshima, M

Nishibe, Y

Hasegawa, M

Yamagishi, A

Oshima, T

机构：

[1] TOKYO UNIV PHARM & LIFE SCI,DEPT MOL BIOL,HACHIOJI,TOKYO 19203,JAPAN

[2] TEIJIN LTD,INST BIOMED RES,HINO,TOKYO 191,JAPAN

[3] INST STAT MATH,MINATO KU,TOKYO 106,JAPAN

来源：

GENE | 1996年 / 180卷 / 1-2期

关键词：

archaea; phylogeny; evolution; rRNA; acidophile; thermophile;

D O I：

10.1016/S0378-1119(96)00451-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A hyperthermophile NC12 was newly isolated from Noboribetsu hot spring. To characterize this organism, a gene coding for 16S rRNA was cloned and sequenced. The 16S rRNA sequence from NC12 shows the highest similarity with those from Pyrodictium occultum and Desulfurococcus mobilis among the sequences in the database, indicating that NC12 belongs to a cluster of extreme thermophiles (Crenarchaeota) in the archaeal domain. However, since the highest identity score was only 91.2%, it is suggested that NC12 may constitute a new genus.

引用

页码：183 / 187

页数：5

共 15 条

[1] ROOTING THE ARCHAEBACTERIAL TREE - THE PIVOTAL ROLE OF THERMOCOCCUS-CELER IN ARCHAEBACTERIAL EVOLUTION [J].