Singlet molecular oxygen triggers the soxRS regulon of Escherichia coli

被引:17
作者
Agnez-Lima, LF
Di Mascio, P
Demple, B
Menck, CFM
机构
[1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed, BR-05508900 Sao Paulo, Brazil
[2] Univ Fed Rio Grande do Norte, Dept Biol Cellular & Genet, Ctr Biociencias, BR-59072970 Natal, RN, Brazil
[3] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05513970 Sao Paulo, Brazil
[4] Harvard Univ, Sch Publ Hlth, Dept Canc Cell Biol, Boston, MA 02115 USA
关键词
oxidative stress; singlet oxygen; soxRS regulon; superoxide;
D O I
10.1515/BC.2001.134
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The electronically excited molecular oxygen (singlet oxygen, O-1(2)) can be detrimental to cells in several ways, although recent reports indicate that it may play a role as an intercellular signal in eukaryotes. Here we present evidence that O-1(2), generated by thermodissociation of disodium 3,3'-(1,4-naphthylidene) diproprionate endoperoxide, activates transcription of genes of the soxRS regulon, and that this induction is paralleled by induction of a soxS'::lacZ operon fusion. The inductions were dependent on a functional soxR gene. These data imply that protective responses, such as induction of the soxRS regulon, may be triggered by diverse environmental oxidative stresses, and that O-1(2) may also function as a signal molecule in prokaryotes.
引用
收藏
页码:1071 / 1075
页数:5
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