Transcellular transport of genistein, a soybean-derived isoflavone, across human colon carcinoma cell line (Caco-2)

被引：20

作者：

Oitate, M ^{[1
]}

Nakaki, R ^{[1
]}

Koyabu, N ^{[1
]}

Takanaga, H ^{[1
]}

Matsuo, H ^{[1
]}

Ohtani, H ^{[1
]}

Sawada, Y ^{[1
]}

机构：

[1] Kyushu Univ, Dept Medicopharmaceut Sci, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 8128582, Japan

来源：

BIOPHARMACEUTICS & DRUG DISPOSITION | 2001年 / 22卷 / 01期

关键词：

isoflavone; genistein; Caco-2; cells; transcellular transport; intestinal absorption;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

Genistein, a soybean-derived isoflavone, is thought to have an anticarcinogenic action, but little is known about the cellular mechanisms of its intestinal absorption. This study was designed to investigate the absorption mechanisms of genistein using human colon carcinoma cell line, Caco-2 cells. The apical-to-basolateral transcellular transport of genistein across a Caco-2 cell monolayer was significantly greater than that in the opposite direction. An uptake experiment revealed that cellular uptake of genistein by Caco-2 cells was concentrative. The transcellular transport of genistein was saturable and temperature-dependent, and was inhibited by other flavonoids such as rutin, quercetin, (+)-catechin and (-)-epicatechin. These results suggest that genistein is transported across Caco-2 cells by a carrier-mediated system, located on the apical membrane. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：23 / 29

页数：7

共 37 条

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