Identification of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom)

被引：58

作者：

Asgeirsdóttir, SA ^{[1
]}

de Vries, OMH ^{[1
]}

Wessels, JGH ^{[1
]}

机构：

[1] Univ Groningen, Biomol Sci & Biotechnol Inst, Plant Mol Biol Lab, NL-9751 NN Haren, Netherlands

来源：

MICROBIOLOGY-UK | 1998年 / 144卷

关键词：

Pleurotus ostreatus; hydrophobin; aerial mycelium; fruiting body; developmental regulation;

D O I：

10.1099/00221287-144-11-2961

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Three proteins with characteristic features of class I hydrophobins, designated POH1, POH2 and POH3, were isolated from the basidiomycete Pleurotus ostreatus. Based on N-terminal sequence analyses, their cDNAs were isolated using RT-PCR; the cDNAs and corresponding genes were sequenced and their regulation studied. POH1 is expressed in the fruiting bodies but not in vegetative mycelium. The regulation of POH2 and POH3 is tightly correlated. Both genes are switched off in the fruiting bodies but abundantly expressed in the vegetative mycelium of both monokaryon and dikaryon. POH2 and POH3 were isolated from the culture medium and from aerial hyphae. Co-purified POH2 and POH3 assembled in vitro into a protein membrane with a typical rodlet pattern as found previously with other hydrophobins. Similar structures were detected on the surface of aerial hyphae.

引用

页码：2961 / 2969

页数：9

共 37 条

[1]

[Anonymous], [No title captured]

[2]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[3] Expression of two closely linked hydrophobin genes of Coprinus cinereus is monokaryon-specific and down-regulated by the oid-1 mutation [J].