Note:: Characterization of Vibrio cholerae O139 Bengal isolated from water in Malaysia

被引:7
作者
Son, R [1 ]
Rusul, G
Samuel, L
Yuherman
Senthil, S
Rasip, A
Nasreldin, EH
Nishibuchi, M
机构
[1] Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Biotechnol, Serdang 43400, Selangor, Malaysia
[2] Univ Putra Malaysia, Fac Food Sci & Biotechnol, Dept Food Sci, Serdang 43400, Selangor, Malaysia
[3] Med Res Inst, Kuala Lumpur, Malaysia
[4] Univ Andalas, Padang, Indonesia
[5] Kyoto Univ, Ctr SE Asian Studies, Sakyo Ku, Yoshida, Kyoto, Japan
关键词
D O I
10.1111/j.1365-2672.1998.tb05273.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Four Vibrio cholerae O139 Bengal strains isolated from surface water were characterized by antibiotic resistance, plasmid profile, presence of cholera toxin gene and random amplification of polymorphic DNA (RAPD) analysis, All four strains exhibit multiple resistance towards the antibiotics tested with a multiple antibiotic resistance index of 0.5-0.66, and harboured a 2.0 MDa non-conjugative plasmid. The Vibrio cholerae O139 Bengal were positive for the cholera toxin gene, Antibiotyping and random amplification of polymorphic DNA analysis with four primers proved to be useful in discriminating the isolates, RAPD proved to be more sensitive. These results reveal that there is significant genetic diversity among the Vibrio cholerae O139 Bengal strains studied.
引用
收藏
页码:1073 / 1077
页数:5
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