Choline deficiency induced by Mycoplasma fermentans enhances apoptosis of rat astrocytes

被引：11

作者：

Ben-Menachem, G

Mousa, A

Brenner, T

Pinto, F

Zähringer, U

Rottem, S ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Sch Med, Dept Membrane & Ultrastruct Res, IL-91120 Jerusalem, Israel

[2] Hadassah Univ Hosp, Dept Neurol, Lab Neuroimmunol, IL-91120 Jerusalem, Israel

[3] Res Ctr Borstel, Ctr Med & Biosci, D-23845 Borstel, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 201卷 / 02期

关键词：

choline deficiency; choline uptake; Mycoplasma fermentans; apoptosis; astrocyte;

D O I：

10.1016/S0378-1097(01)00267-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A choline uptake system accumulating free choline in an energy-dependent process is described in Mycoplasma fermentans. The uptake system has a K-m of 2.2 x 10(-5) M and a V-max of 0.15 nmol 10 min(-1) mg(-1) cell protein and the choline incorporated could be recovered in the soluble fraction as free choline, phosphorylcholine and CDP-choline. Choline accumulation by M. fermentans resulted in a marked choline depletion of the growth medium. The choline depletion of an astrocyte cell culture induced by M. fermentans was associated with the apoptotic death of the cells. Apoptosis was not obtained with heat-inactivated mycoplasmas and could be reversed by the addition of free choline to the growth medium. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：157 / 162

页数：6

共 25 条

[1] Choline deficiency induces apoptosis and decreases the number of eosinophilic preneoplastic foci in the liver of OXYS rats [J].