Relationship between the flow-rate of the mobile phase and retention of the stationary phase in counter-current chromatography

被引：68

作者：

Du, QZ

Wu, CJ

Qian, GJ

Wu, PD

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Chinese Acad Agr Sci, Tea Res Inst, Chinesse Minist Agr, Key Lab Tea Chem Engn, Hangzhou 310008, Zhejiang, Peoples R China

[3] Zhejiang Univ, Dept Chem Engn, Hangzhou 310027, Zhejiang, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 835卷 / 1-2期

关键词：

counter-current chromatography; flow-rate; retention of stationary phase;

D O I：

10.1016/S0021-9673(98)01066-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The retention percentages of the stationary phase (S-F) at various flow-rates (F-c) of 15 two-phase solvent systems were determined in three types of counter-current chromatographic apparatus equipped with small-, middle- and large-bore coiled columns. The regression analysis of S-F showed a linear relationship between the square root of the flow-rate and the retention percentage of the stationary phase, i.e. S-F=A-B root F-C where A indicates the difference in the solvent composition of the solvent system and B indicates the difference in the volume ratio of the solvents for the same composition of the solvent system. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：231 / 235

页数：5