The molecular scanner:: concept and developments

被引:24
作者
Binz, PA
Müller, M
Hoogland, C
Zimmermann, C
Pasquarello, C
Corthals, G
Sanchez, JC
Hochstrasser, DF
Appel, RD
机构
[1] CMU, Proteome Informat Grp, Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland
[2] Univ Geneva, Fac Med, CH-1211 Geneva, Switzerland
[3] Univ Hosp Geneva, Biomed Proteom Res Ctr, CH-1211 Geneva, Switzerland
关键词
D O I
10.1016/j.copbio.2003.12.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry and dedicated bioinformatics have been assembled in the development of the molecular scanner. This highly flexible and data-rich approach has combined the power of electrophoretic protein separation, the simultaneous digestion and transfer of proteins through an enzymatic membrane, the immediate use of the MALDI mass spectrometer to scan a collecting membrane, and the development of dedicated bioinformatics tools to perform protein identification and molecular imaging of the proteome. Clinical applications of the molecular scanner have also started to be developed for disease diagnosis in biological material.
引用
收藏
页码:17 / 23
页数:7
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