Ion liquid chromatography on-a-chip with beads-packed parylene column

被引：14

作者：

He, Q ^{[1
]}

Pang, CL ^{[1
]}

Tai, YC ^{[1
]}

Lee, TD ^{[1
]}

机构：

[1] CALTECH, Caltech Micromachining Lab, Pasadena, CA 91125 USA

来源：

MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST | 2004年

关键词：

D O I：

10.1109/MEMS.2004.1290560

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A parylene-MEMS ion-exchange Liquid Chromatography (LC) chip is presented here. The chip is integrated with microfluidic I/O ports, a separation column, frits/filters, and a conductivity detector. The column is packed with conventional LC stationary phase support materials, i.e. micro-beads with surface functional groups. To withstand high pressure normally encountered in High Performance Liquid Chromatography (HPLC), a self-aligned, channel-anchoring technique is developed to increase the pressure rating of the Parylene microfluidic devices from 30 to at least 800psi. On-chip injection, separation and detection of anions in water, with similar to25ppm concentration, have been successfully demonstrated. To our knowledge, this is the first demonstration of microbeads-packed column ion liquid chromatography (LC) on a chip.

引用

页码：212 / 215

页数：4

共 10 条

[1] A closed-loop controlled electrochemically actuated micro-dosing system [J].

Böhm, S ;

Timmer, B ;

Olthuis, W ;

Bergveld, P .

JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2000, 10 (04) :498-504

[2] An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases [J].

Ceriotti, L ;

de Rooij, NF ;

Verpoorte, E .

ANALYTICAL CHEMISTRY, 2002, 74 (03) :639-647

[3] Fabrication of nanocolumns for liquid chromatography [J].

He, B ;

Tait, N ;

Regnier, F .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3790-3797

[4]

LIGER M, MEMS 03, P602

[5] DESIGN OF AN OPEN-TUBULAR COLUMN LIQUID CHROMATOGRAPH USING SILICON CHIP TECHNOLOGY [J].

MANZ, A ;

MIYAHARA, Y ;

MIURA, J ;

WATANABE, Y ;

MIYAGI, H ;

SATO, K .

SENSORS AND ACTUATORS B-CHEMICAL, 1990, 1 (1-6) :249-255

[6]

Meyer V.R., 1999, PRACTICAL HIGH PERFO, P310

[7] Ion chromatography on-chip [J].

Murrihy, JP ;

Breadmore, MC ;

Tan, AM ;

McEnery, M ;

Alderman, J ;

O'Mathuna, C ;

O'Neill, AP ;

O'Brien, P ;

Advoldvic, N ;

Haddad, PR ;

Glennon, JD .

JOURNAL OF CHROMATOGRAPHY A, 2001, 924 (1-2) :233-238

[8] On-chip definition of picolitre sample injection plugs for miniaturised liquid chromatography [J].

O'Neill, AP ;

O'Brien, P ;

Alderman, J ;

Hoffman, D ;

McEnery, M ;

Murrihy, J ;

Glennon, JD .

JOURNAL OF CHROMATOGRAPHY A, 2001, 924 (1-2) :259-263

[9]

OCVIRK G, 1995, ANAL METHOD INSTRUM, V2, P74

[10]

SINGH AK, 2003, P MUTAS, P1163

← 1 →