Controlled encapsulation of single-cells into monodisperse picolitre drops

被引：413

作者：

Edd, Jon F. ^{[1
]}

Di Carlo, Dino ^{[1
]}

Humphry, Katherine J. ^{[2
,3
]}

Koster, Sarah ^{[2
,3
]}

Irimia, Daniel ^{[1
]}

Weitz, David A. ^{[2
,3
]}

Toner, Mehmet ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, BioMEMS Resource Ctr, Charlestown, MA USA

[2] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

来源：

LAB ON A CHIP | 2008年 / 8卷 / 08期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1039/b805456h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation.

引用

页码：1262 / 1264

页数：3

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