Gamma interferon-based Immunodiagnosis of tuberculosis:: Comparison between whole-blood and enzyme-linked immunospot methods

被引：39

作者：

Schölvinck, E

Wilkinson, KA

Whelan, AO

Martineau, AR

Levin, M

Wilkinson, RJ

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Div Med, London W2 1PG, England

[2] Univ London Imperial Coll Sci Technol & Med, Fac Med, Wellcome Ctr Res Clin Trop Med, London W2 1PG, England

[3] Vet Labs Agcy, Weybridge KT15 3NB, Surrey, England

[4] Northwick Pk Hosp & Clin Res Ctr, Tuberculosis Serv, Harrow HA1 3UJ, Middx, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 02期

关键词：

D O I：

10.1128/JCM.42.2.829-831.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gamma interferon secretion levels of 52 adults whose T cells had been stimulated with the Mycobacterium tuberculosis antigens CFP-10 and ESAT-6 were measured by enzyme-linked immunospot methods and a whole-blood-based assay. The test results correlated well (r = 0.689, P < 0.0001). Its simple format and use of only small volumes of blood make the whole-blood assay suitable for pediatric practice and field trials.

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页码：829 / 831

页数：3

相关论文

共 23 条

[1] Genomic approach to identification of Mycobacterium bovis diagnostic antigens in cattle [J].

Aagaard, C ;

Govaerts, M ;

Okkels, LM ;

Andersen, P ;

Pollock, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (08) :3719-3728

[2] Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M-kansasii [J].

Arend, SM ;

van Meijgaarden, KE ;

de Boer, K ;

de Palou, EC ;

van Soolingen, D ;

Ottenhoff, THM ;

van Dissel, JT .

JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (12) :1797-1807

[3] Detection of active tuberculosis infection by T cell responses to early-secreted antigenic target 6-kDa protein and culture filtrate protein 10 [J].

Arend, SM ;

Andersen, P ;

van Meijgaarden, KE ;

Skjot, RLV ;

Subronto, YW ;

van Dissel, JT ;

Ottenhoff, THM .

JOURNAL OF INFECTIOUS DISEASES, 2000, 181 (05) :1850-1854

[4] Diagnosing latent tuberculosis infection - The 100-year upgrade [J].

Barnes, PF .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 163 (04) :807-808

[5] Comparative genomics of BCG vaccines by whole-genome DNA microarray [J].

Behr, MA ;

Wilson, MA ;

Gill, WP ;

Salamon, H ;

Schoolnik, GK ;

Rane, S ;

Small, PM .

SCIENCE, 1999, 284 (5419) :1520-1523

[6] Evaluation of a whole-blood interferon-γ release assay for the detection of Mycobacterium tuberculosis infection in 2 study populations [J].

Bellete, B ;

Coberly, J ;

Barnes, GL ;

Ko, C ;

Chaisson, RE ;

Comstock, GW ;

Bishai, WR .

CLINICAL INFECTIOUS DISEASES, 2002, 34 (11) :1449-1456

[7] Gamma interferon responses induced by a panel of recombinant and purified mycobacterial antigens in healthy, non-Mycobacterium bovis BCG-vaccinated Malawian young adults [J].

Black, GF ;

Weir, RE ;

Chaguluka, SD ;

Warndorff, D ;

Crampin, AC ;

Mwaungulu, L ;

Sichali, L ;

Floyd, S ;

Bliss, L ;

Jarman, E ;

Donovan, L ;

Andersen, P ;

Britton, W ;

Hewinson, G ;

Huygen, K ;

Paulsen, J ;

Singh, M ;

Prestidge, R ;

Fine, PEM ;

Dockrel, HM .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2003, 10 (04) :602-611

[8] BCG-induced increase in interferon-gamma response to mycobacterial antigens and efficacy of BCG vaccination in Malawi and the UK: two randomised controlled studies [J].

Black, GF ;

Weir, RE ;

Floyd, S ;

Bliss, L ;

Warndorff, DK ;

Crampin, AC ;

Ngwira, B ;

Sichali, L ;

Nazareth, B ;

Blackwell, JM ;

Branson, K ;

Chaguluka, SD ;

Donovan, L ;

Jarman, E ;

King, E ;

Fine, PEM ;

Dockrell, HM .

LANCET, 2002, 359 (9315) :1393-1401

[9]

Brock I, 2001, INT J TUBERC LUNG D, V5, P462

[10] Rapid detection of active and latent tuberculosis infection in HIV-positive individuals by enumeration of Mycobacterium tuberculosis-specific T cells [J].

Chapman, ALN ;

Munkanta, M ;

Wilkinson, KA ;

Pathan, AA ;

Ewer, K ;

Ayles, H ;

Reece, WH ;

Mwinga, A ;

Godfrey-Faussett, P ;

Lalvani, A .

AIDS, 2002, 16 (17) :2285-2293