The functional regeneration of syncytiotrophoblast in cultured explants of term placenta

被引:107
作者
Simán, CM
Sibley, CP
Jones, CJP
Turner, MA
Greenwood, SL
机构
[1] Univ Manchester, St Marys Hosp, Acad Unit Child Hlth, Manchester M13 0JH, Lancs, England
[2] Univ Manchester, St Marys Hosp, Acad Unit Obstet & Gynaecol, Manchester M13 0JH, Lancs, England
关键词
explant culture; potassium transport; angiotensin II; adenosine 5 '-triphosphate; osmolality;
D O I
10.1152/ajpregu.2001.280.4.R1116
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
We have investigated the functional characteristics of term human placental villous explants kept in long-term (7-11 days) culture. Fragments of placental villous tissue ( similar to5-10 mg wet wt) were cultured in supplemented CMRL-1066 culture medium for up to 11 days. After the first day of culture, the syncytiotrophoblast appeared vacuolated and eventually degenerated. However, a new syncytiotrophoblast developed by day 4, being indistinguishable from that of a fresh placenta by 11 days. Release of human chorionic gonadotrophin increased and activity of lactate dehydrogenase in culture medium decreased with culture time. Transport variables were measured over the first 7 days of culture. Basal Rb-86 efflux was reduced with time in culture and was inhibited by Ba2+, suggesting the efflux was mediated by K+ channels. At all stages of culture, Rb-86 efflux was stimulated by ATP, hyposmotic medium, and ANG II. A complex pattern of efflux changes with culture time and type of stimulator was observed, suggesting that several compartments of the tissue contributed to stimulated efflux. This culture system provides opportunities for studies of chronic regulation of placental function.
引用
收藏
页码:R1116 / R1122
页数:7
相关论文
共 31 条
[1]  
BIRDSEY TJ, 1999, AM J PHYSL REGULATOR, V276
[2]  
Boyd J D, 1967, J Obstet Gynaecol Br Commonw, V74, P161
[3]  
Boyd R, 1997, TROPHOBLAST RES, V9, P75
[4]  
Clarson LH, 2000, J PHYSIOL-LONDON, V528P, p20P
[5]   GESTATIONAL CHANGES IN CA-2+ TRANSPORT ACROSS RAT PLACENTA AND MESSENGER-RNA FOR CALBINDIN9K AND CA2+-ATPASE [J].
GLAZIER, JD ;
ATKINSON, DE ;
THORNBURG, KL ;
SHARPE, PT ;
EDWARDS, D ;
BOYD, RDH ;
SIBLEY, CP .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (04) :R930-R935
[6]  
HAUSSINGER D, 1994, AM J PHYSIOL-ENDOC M, V267, pE345
[7]   COMPARISON OF HISTOLOGICAL CHANGES SEEN IN PLACENTAL TISSUE-CULTURES AND IN PLACENTAE OBTAINED AFTER FETAL DEATH [J].
HUSTIN, J ;
GASPARD, U .
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 1977, 84 (03) :210-215
[8]   SUBSTRATE-SPECIFIC REGULATION OF THE TAURINE TRANSPORTER IN HUMAN PLACENTAL CHORIOCARCINOMA CELLS (JAR) [J].
JAYANTHI, LD ;
RAMAMOORTHY, S ;
MAHESH, VB ;
LEIBACH, FH ;
GANAPATHY, V .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1995, 1235 (02) :351-360
[9]   ULTRASTRUCTURE OF THE NORMAL HUMAN PLACENTA [J].
JONES, CJP ;
FOX, H .
ELECTRON MICROSCOPY REVIEWS, 1991, 4 (01) :129-178
[10]   INSULIN-LIKE GROWTH-FACTOR-I STIMULATES AMINO-ACID-UPTAKE BY THE CULTURED HUMAN PLACENTAL TROPHOBLAST [J].
KARL, PI .
JOURNAL OF CELLULAR PHYSIOLOGY, 1995, 165 (01) :83-88